Wang Lab

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Organoid

The current research in Wang Lab focuses on tissue engineering and regenerative medicine using organoids. As PI or co-Investigator on several universities- and foundation-funded grants, Dr. Wang laid the groundwork for the proposed research by developing collagen I based gels for the culture of primary intestinal stem cells and use collagen I foam as cell carriers for the delivery of intestinal organoids [1], and by demonstrating the feasibility to encapsulate DNA-functionalized gold nanoparticles and drug-loaded PLGA nanoparticles [2] into primary isolated intestinal intestinal organoids to form an intestinal Trojan horse for gene regulation therapy of inflammatory bowel disease [3]. Dr. Wang demonstrated that the external perturbations in the lumen, like engineered nanomaterials [4, 5] and dietary interventions [6, 7] controlled the proliferation and differentiation of intestinal organoids ex vivo. Most interestingly, the miniguts treated with 200 ng/ml RANKL for 3 days were immunostained with two M-cells specific marker: GP2 (green) and UEA-1(red), which confirmed the successful inducing of intestinal organoids into M-cells [8]. He further confirmed that the intestinal organoids from different microbiota environment demonstrated the concentration-dependent responses when they are exposed to inflammatory stimulants [9]. Dr. Wang is aware the current major non-genetic cell engineering strategies for drug delivery and cell-based therapies [10, 11].

The morphology of intestinal stem cells cultured in Matrigel during 7 days. The scale bars were 200 μm [2].

The morphology of an intestinal organoid cultured in Matrigel during 7 days. The scale bars were 200 μm [2].


1) Peng H, Poovaiah N, Forrester M, Cochran E, Wang Q. Ex vivo culture of primary intestinal stem cells in collagen gels and foams. ACS Biomaterials Science & Engineering. 2015; 1(1):37-42.
2) Peng H, Wang C, Xu X, Yu C, Wang Q. An intestinal Trojan horse for gene delivery. Nanoscale. 2015; 7(10):4291-305. PMID: 25619169

3) Davoudi Z, Peroutka-Bigus N, Bellaire B, Wannemuehler M, Barrett TA, Narasimhan B, Wang Q. Intestinal Organoids Containing PLGA Nanoparticles for the Treatment of Inflammatory Bowel Diseases. Journal of biomedical materials research. Part A. 2018; 106(4): 876-886. PMID: 29226615

4) Qi Y, Shi E, Peroutka-Bigus N, Bellaire B, Wannemuehler M, Jergens A, Barrett T, Wu Y, Wang Q. Ex vivo Study of Telluride Nanowires in Minigut. Journal of biomedical nanotechnology. 2018; 14(5): 978-986. PMID: 29883567

5) Reding B, Carter P, Qi Y, Li Z, Wu Y, Wannemuehler M, Bratlie KM, Wang Q. Manipulate intestinal organoids with niobium carbide nanosheets. J Biomed Mater Res A. 2020 Jun 7; doi: 10.1002/jbm.a.37032. PMID: 32506610.

6) Cai T, Qi Y, Jergens A, Wannemuehler M, Barrett TA, Wang Q. Effects of Six Common Dietary Nutrients on Murine Intestinal Organoid Growth. PloS one. 2018; 13(2): e0191517. PMID: 29389993

7) Qi Y, Lohman J, Bratlie KM, Peroutka-Bigus N, Bellaire B, Wannemuehler M, Yoon KJ, Barrett TA, Wang Q. Vitamin C and B3 as New Biomaterials to Alter Intestinal Stem Cells. Journal of biomedical materials research. Part A. 2019;107(9):1886-1897. PMID: 31071241

8) Tong T, Qi Y, Bussiere LD, Wannemuehler M, Miller CL, Wang Q, Yu C. Transport of Artificial Virus-like Nanocarriers (AVN) through Intestinal Monolayer via Microfold Cells. Nanoscale. 2020;12(30): 16339-16347. PubMed PMID: 32725029.

9) Sun L, Rollins D, Qi Y, Fredericks J, Mansell TJ, Jergens A, Phillips GJ, Wannemuehler M, Wang Q. TNFα Regulates Intestinal Organoids from Mice with Both Defined and Conventional Microbiota. International journal of biological macromolecules. 2020; 164(1): 548-556. PMID: 32693143.

10) Wang Q, Cheng H, Peng H, Zhou H, Li PY, Langer R. Non-genetic engineering of cells for drug delivery and cell-based therapy. Advanced Drug Delivery Reviews. 2015; 91(1):125-140. PMID: 25543006
11) Ikoba U, Peng H, Li H, Miller C, Yu C, Wang Q. Nanocarriers in therapy of infectious and inflammatory diseases. Nanoscale. 2015; 7(10):4291-305. PMID: 25680099


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