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in the new paper, “Organoids“, published in Nature Reviews, Dr. Wang and other leaders in organoid research highlight the rationale underlying the establishment of organoid cultures and provide guiding principles for selecting suitable materials, methods, and protocols for different applications. The critical considerations for generating robust organoids have been further discussed, such as those related to cell isolation and seeding, matrix and soluble factor selection, physical cues and integration (see Graphic Abstract below), as well as the general standards for data quality, reproducibility and deposition within the organoid community. It is a good resource for Methods and Protocols if you want to make organoids by yourself.

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